Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid: experimental studies in thromboelastometry

Abstract Background Colloid fluids supplemented with adequate combinations of coagulation factor concentrates the capability to restore could be a desirable future treatment component in massive transfusion. Methods Starting from and blood cell-free albumin solution we added Prothrombin Complex Concentrate, Fibrinogen Concentrate Factor XIII different concentrations analyze their properties thromboelastometry parameters without use plasma. Further analysis under presence platelets was performed for comparability whole conditions. Results Albumin solutions enriched at optimized show restoring potential. showed sufficient thrombin formation inducing fibrinogen polymerization. The combination led stable vitro fibrin clot. excellent capacity improve clot firmness expressed as Amplitude 10 min Maximal Clot Firmness. alone, or XIII, able normal Firmness values. In platelets, surrogate parameter generation (Clotting Time) improves normalizes when compared blood. Conclusions Combinations suspended can absence This kind artificial colloid coagulation-restoring characteristics might offer new alternatives Trial registration Study registered institutional ethic committee “Institut de Recerca, Hospital Santa Creu i Sant Pau, protocol number IIBSP-CFC-2013-165.